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(A) Schematic of neonatal viral injection (P0–P2) delivering Cre recombinase and an mCherry reporter (AAV-eSYN-mCherry-T2A-iCre-WPRE) or EGFP reporter (AAV-eSYN-EGFP-T2A-iCre-WPRE) to excitatory neurons in primary visual cortex (V1) of NR1 flox/flox mice. Created in BioRender. Rannio, S. (2026) https://BioRender.com/27fmwdc (B) Representative confocal image showing sparse mCherry expression in V1 at P21, including labeling of a few L5 PCs with easily identified by their prominent apical dendrites.

Journal: bioRxiv

Article Title: Distinct NMDA Receptor Pools Determine Diverse Forms of Cortical Plasticity

doi: 10.64898/2026.02.14.705915

Figure Lengend Snippet: (A) Schematic of neonatal viral injection (P0–P2) delivering Cre recombinase and an mCherry reporter (AAV-eSYN-mCherry-T2A-iCre-WPRE) or EGFP reporter (AAV-eSYN-EGFP-T2A-iCre-WPRE) to excitatory neurons in primary visual cortex (V1) of NR1 flox/flox mice. Created in BioRender. Rannio, S. (2026) https://BioRender.com/27fmwdc (B) Representative confocal image showing sparse mCherry expression in V1 at P21, including labeling of a few L5 PCs with easily identified by their prominent apical dendrites.

Article Snippet: NR1 flox/flox pups P0-2 were injected with AAV-eSYN-mCherry-T2A-iCre-WPRE (VB4856, Vector Biolabs) or AAV-eSYN-EGFP-T2A-iCre-WPRE (VB4855, Vector Biolabs) into V1 using the following coordinates (in mm, relative to lambda): medial-lateral ± 1.10; anterior-posterior 0.

Techniques: Injection, Expressing, Labeling

(A) Schematic of neonatal viral injection (P0–P2) delivering Cre recombinase and an mCherry reporter (AAV-eSYN-mCherry-T2A-iCre-WPRE) or EGFP reporter (AAV-eSYN-EGFP-T2A-iCre-WPRE) to excitatory neurons in primary visual cortex (V1) of NR1 flox/flox mice. Created in BioRender. Rannio, S. (2026) https://BioRender.com/27fmwdc (B) Representative confocal image showing sparse mCherry expression in V1 at P21, including labeling of a few L5 PCs with easily identified by their prominent apical dendrites.

Journal: bioRxiv

Article Title: Distinct NMDA Receptor Pools Determine Diverse Forms of Cortical Plasticity

doi: 10.64898/2026.02.14.705915

Figure Lengend Snippet: (A) Schematic of neonatal viral injection (P0–P2) delivering Cre recombinase and an mCherry reporter (AAV-eSYN-mCherry-T2A-iCre-WPRE) or EGFP reporter (AAV-eSYN-EGFP-T2A-iCre-WPRE) to excitatory neurons in primary visual cortex (V1) of NR1 flox/flox mice. Created in BioRender. Rannio, S. (2026) https://BioRender.com/27fmwdc (B) Representative confocal image showing sparse mCherry expression in V1 at P21, including labeling of a few L5 PCs with easily identified by their prominent apical dendrites.

Article Snippet: NR1 flox/flox pups P0-2 were injected with AAV-eSYN-mCherry-T2A-iCre-WPRE (VB4856, Vector Biolabs) or AAV-eSYN-EGFP-T2A-iCre-WPRE (VB4855, Vector Biolabs) into V1 using the following coordinates (in mm, relative to lambda): medial-lateral ± 1.10; anterior-posterior 0.

Techniques: Injection, Expressing, Labeling